Device

Part:BBa_K1946004:Design

Designed by: Ayşe Naz Ozantürk   Group: iGEM16_Bilkent-UNAMBG   (2016-10-19)


Toluene/Xylene Sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2248
    Illegal BamHI site found at 1751
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1168
    Illegal BsaI.rc site found at 1546
    Illegal SapI.rc site found at 2266


Design Notes

XylR (BBa_K1946005) in this composite part was codon optimized by us for E.coli K12.


Source

The source of XylR (BBa_K1946005) can be found at GenBank: AAB59162.1 . pu promoter (BBa_K1946006) was synthetically synthesized from GeneWiz based on Pseudomonas putida TOL plasmid. sfGFP sequence can be accessed in the registry at BBa_I746916.

References

Huang WE, Singer AC, Spiers AJ, Preston GM, Whiteley AS. (2008) Characterizing the regulation of the Pu promoter in Acinetobacter baylyi ADP1. Environ Microbiol. 2008;10(7):1668-80.

Zhang Y-T, Jiang F, Tian Z-X, Huo Y-X, Sun Y-C, et al. (2014) CRP-Cyclic AMP Dependent Inhibition of the Xylene-Responsive s54-Promoter Pu in Escherichia coli. PLoS ONE 9(1): e86727. doi:10.1371/journal.pone.0086727